Solution Nuclear Magnetic Resonance Spectroscopy Techniques for Probing Intermolecular Interactions

Protein structure determination in solution by nuclear magnetic resonance spectroscopy.

Knowledge of three-dimensional protein structures is one of the foundations of protein design and protein engineering. Nuclear magnetic resonance spectroscopy was recently introduced as a second method for protein structure determination, in addition to the well-established diffraction techniques with protein single crystals. This new approach enables one to carry out detailed structural studie...

Proton Nuclear Magnetic Resonance Spectroscopy

18. Nuclear Magnetic Resonance Spectroscopy

Nuclear Magnetic Resonance (NMR) is a spectroscopic technique widely used by organic chemists. NMR provides details about the location, number and environment of specific types of atoms within a sample. The proper interpretation of this information allows detailed chemical structures to be discerned. The same basic instrumentation used in this week’s experiment is used in physician offices when...

Effects of Intermolecular Interactions and Intramolecular Dynamics on Nuclear Resonance

There are several facets to our interest in temperature, solvent, and isotope effects on NMR chemical shifts. Excepting molecular beam studies, the application of NMR to the elucidation of molecular structure and mechanisms of molecular reactions nearly always involves observations of molecules in some environment. Before NMR can be used to obtain structural and mechanistic information unambigu...

Nuclear Magnetic Resonance Spectroscopy in Macromolecular Science

Today, virtually every experimental paper published in ‘‘Macromolecular Chemistry and Physics’’ includes characterization of the polymers under study by nuclear magnetic resonance (NMR) spectroscopy. In addition to monitoring the various synthetic steps, which eventually lead to the new polymers or supramolecular systems described, high-resolution NMR provides a number of important parameters, ...